Durum wheat is highly susceptible to Fusarium head blight (FHB), a destructive fungal disease mainly caused by Fusarium graminearum. It has narrow genetic variation for FHB resistance, and breeding programs have been challenged by the lack of reliable resistance sources within the elite gene pool. Moreover, studies have indicated the presence of susceptibility genes/factors in the durum wheat genome, which may further make the crop more susceptible to FHB. Generating mutant populations and analyzing the effect of gene mutations is a useful approach for identifying novel genes involved in plant disease susceptibility. A durum wheat mutant population was previously developed through EMS treatment of the cultivar ‘Kronos’ and characterized by exome capture and resequencing to detect the mutated genes. In this study, we evaluated 500 M4 mutants from this population across multiple greenhouse experiments to identify candidate genes involved in FHB susceptibility in durum wheat. Our results showed significant differences in FHB severity among the examined Kronos mutants, ranging from resistant to completely susceptible. We identified 11 mutant lines that consistently showed more resistance with average disease severity (DS) ≤ 33% to FHB compared to the wildtype Kronos (average DS = 70%). Analysis of all genes mutated in the selected resistant mutants using the information obtained from the wheat TILLING database (https://dubcovskylab.ucdavis.edu/wheat-tilling) identified 22 genes with non-synonymous or nonsense mutations shared across at least three resistant mutants. Our ongoing work focuses on knocking out the identified candidate genes in durum wheat through the wheat × maize hybridization coupled with genome editing method that has been established in our lab. This research will provide new insights into genes related to FHB susceptibility and facilitate breeding durum wheat cultivars with improved FHB resistance.