Fusarium Head Blight of wheat and barley is a complex and ever evolving disease. Resistance to fungal progression is under the control of multiple plant genes of varying effectiveness. The major QTL FHB1 provides the best resistance to fungal progression, although it does not appear to facilitate resistance to initial infection nor is the consequence of FHB1 expression a complete elimination of FHB spread. Novel sources of FHB resistance are in constant need and the identification and characterisation of sources of susceptibility may also aid in the development of more stable resistant wheat and barley lines. Over the past 3 years our lab has screened through roughly 400 lines of an EMS mutagenized family of wheat cultivar Kronos and another roughly 3000 lines of an EMS mutagenized family of barley cultivar Conlon. From that initial set of mutagenized Kronos lines, we have identified 30 lines as being more susceptible and another 25 lines as being more resistant than the parental line. These mutagenized Kronos lines have been retested to verify their phenotype and we have identified 12 lines which are reproducibly susceptible and another seven which are reproducibility resistant to FHB. To identify the map locations of the increased resistance, we are developing populations from the crosses of the resistant  lines to the wheat cultivar Langdon. We have also initiated a search for Kronos lines with predicted deleterious mutations in metabolic pathway genes known to be up-regulated during Fusarium graminearum infection. Similarly, we identified 45 and 83 lines of the mutagenized Conlon family as being either more resistant or more susceptible than the controls, respectively. These resistant and susceptible lines will be retested in the summer of 2025 to verify their phenotype and to begin narrowing down potential target lines for further analysis.