While exosomes are known to play critical roles in intercellular communication and immune responses during plant-pathogen interactions, their involvement in the cereal-Fusarium interaction particularly within the reproductive tissue apoplast, remains largely unexplored.  Previous research in the lab investigated the impact of Fusarium graminearum (F.g.) on leaf apoplastic fluid changes in barley during F.g. infection.  That work revealed the induction of pathogenesis-related (PR) proteins in the apoplast of infected tissue, proteins such as chitinases, nsLTPs, thaumatin-like proteins, Bowman–Birk-type trypsin inhibitors, and leucine-rich repeat proteins. Here we investigate the possibility of isolating exosomes from the apoplast fluid isolated from infected barley reproductive tissue.  This work revealed that 20 grams of fresh weight barley spike material is adequate for the isolation of apoplastic fluid proteins (P40) and 2-5X that amount is required for purified exosomes (iodixanol gradient) from that same fluid.  Proteomic results collected by spectral scanning mass spectrometry (MS) will be presented. By specifically targeting exosomes collected from the reproductive tissue apoplastic fluid, this research reveals a complex interplay between barley and F.g. during a critical stage of infection and may provide insights into novel targets for disease resistance.