Protocols, Tips and Tricks
Seed Cleaning | Inoculum Production/Media Recipes | Isolates
General
- 2022 NFHB Forum Nuts and Bolts Presentation in PDF Format (Includes information on growing Fusarium graminearum)
DON Sampling/Analysis
- Sampling Grain for Deoxynivalenol (DON) Analysis: A Researchers Guide - Questions? Contact: Ruth Dill-Macky, UMN, St. Paul, MN
- Grain Sampling for DON Analysis (North Dakota State University)
Fusarium Damaged Kernels (FDK)
- A Visual Scale to Estimate Severity of Fusarium Head Blight in Wheat (North Dakota State University)
- Quality Parameters in Small Grains from Minnesota Affected by Fusarium Head Blight
Greenhouse Screening
- Protocols - Type 1 Resistance - Questions? Contact: Christina Cowger, USDA-ARS, North Carolina
- Protocol - Questions? Contact: Guihua Bai, USDA-ARS, Kansas
- Protocol (University of Kentucky)
Field Screening
- Protocol (University of Kentucky)
- Methods for FHB screening nurseries including inoculum production (Cereal Research Centre, Canada) Gilbert, J., Woods, S.M. 2006. "Strategies and Considerations for Multi-Location FHB Screening Nurseries." In: Ban, T., J.M. Lewis, and E.E. Phipps (eds.) The Global Fusarium Initiative for International Collaboration: A Strategic Planning Workshop held at CIMMYT, El Batán, Mexico; March 14 - 17, 2006. Mexico, D.F.: CIMMYT., pp. 93-102
AUDPC Screening
- Protocol (University of Kentucky)
Inoculum Production/Media Recipes
- Inoculum production methods used by Wheat Breeding Program at University of Illinois
- Conidia Production - Questions? Contact: Guihua Bai, USDA-ARS, Kansas
- Inoculum production methods used by Wheat Breeding Program at University of Kentucky
Isolates
- Isolating F. graminearum from Scabby Seed (Univ. of Illinois)
- Isolate Collection (University of Kentucky)
- Isolating Gibberella zeae from Wheat Heads - Questions? Contact: David Schmale, Virginia Tech.