A winter barley scab evaluation nursery was planted at multiple locations across North America the 2021–22 and 2022–23 field seasons. This trial is referred to as the winter North American Barley Scab Evaluation Nursery (NABSEN). Six North American public breeders submitted lines for inclusion the 2021–22 season, and seven the 2022–23 season. The nursery was planted at seven locations 2021–22 and eight locations 2022–23. Disease pressure was significantly higher at the Blacksburg Virginia and Wooster Ohio locations both seasons over that of the other trial locations. Comparison of DON levels between the Blacksburg and Wooster locations for the individual lines indicated a significant correlation for the two trial years. Ranking the lines for DON accumulation using only the Ohio and Virginia data was carried out, which showed stratification of the lines. A question raised was whether lines were escaping infection as a consequence of heading date, for example by avoiding high disease pressure when environmental conditions are most favorable for disease progression. To address this question, a correlation test and an analysis of variance (ANOVA) was carried out. Neither of these analytical methods supported there being a strong correlation between DON levels and heading date. However the ANOVA suggested the possibility of a correlation between DON levels of the line and the program from which the line came from; a preponderance of low DON accumulating lines emanated from the University of Minnesota and Virginia Tech breeding programs. Thank you to Drs. Pierce Paul and Wanderson Moraes for providing the corn spawn and F. graminarum spore inoculum and for your guidance and help with inoculations, to all NABSEN participants planting the nursery and carrying out all steps to obtain the data, and to Dr. David Francis for providing help with some of the statistical analyses. This work is supported by the United States Department of Agriculture and the U.S. Wheat and Barley Scab Initiative. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0206-0-174, and USWBSI Project ID FY22‐BA‐006. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.