Increasing Fusarium Head Blight (FHB) disease on barley as a result of changes in climate and maize cultivation practices is placing the Nation's high-quality malt supply at risk of being inadequate. At the same time the Independent Craft Brewing Movement, which is undergoing exponential growth, is seeking locally-produced barley. These trends are upending traditional systems. As a consequence breeders across North America must develop portfolios of barley varieties, each resistant to FHB and adapted to its own unique environment. In autumn 2020 we planted the first FHB (scab) nursery for winter barley in Ohio. Included in the planting is a population of ~170 lines. This set consists of highly diverse two-row winter barley lines associated with our breeding program. We also planted a population of 82 recombinant lines from the 95SR316A × Charles cross, which we are referring to as the “Bregitzer population.” Each line was planted in three reps. Also included were a set of six lines previously shown to differ in disease incidence and DON accumulation. Combined, we are testing ~260 different barley lines. Phenotypic data for the ~260 lines were obtained in the form of a visual fusarium incidence score. We are preparing samples for DON and related toxin level determinations at present. Each of the 170 lines were genotyped using a multiplexed sequencing (GMS) platform, and a subset using the Barley 50k iSelect SNP Array. The phenotypic data will then be tested for associations with regions of the barley genome, with the long-term goal being to utilize this information to select for resistances at the genotypic level. A subset of lines exhibiting low disease incidence have also been crossed to Ohio-adapted winter-hardy lines. Acknowledgements: Salaries and research support for E. J. Stockinger and B. Eggers were provided by state and federal funds to the Ohio Agricultural Research and Development Center. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement No. 59-0206-0-174. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture.