Fusarium head blight (FHB) disease in wheat and barley is caused by Fusarium species. Genetic mapping has identified many quantitative trait loci (QTL) contributing to host resistance to FHB. Mapping studies utilizing ‘Chevron’, a six-rowed resistant landrace originated from Switzerland, have consistently detected QTL on chromosome 2H in barley, which co-localized with a heading date QTL. The aims of the present project are to (1) develop recombinant near-isogenic lines (rNILs) for the 2H QTL region and characterize their disease and correlated agronomic phenotypes; and (2) fine-map the QTL and identify candidate genes. To fine map the 2H QTL, an F₂ population of 2,038 plants was generated from a cross of ‘Gen1-001’ (resistant parent, 2H QTL region derived from Chevron introgressed into M69) to ‘M69’ (a susceptible breeding line) and was genotyped with SNP markers flanking the introgressed region (~26 cM).  A total of 489 recombinants were identified which were further genotyped with 32 SNP markers spanning the introgression. This resulted in 17 recombinant classes and homozygous F_2:3 plants were identified. F_2:3 and F_2:4 plants were phenotyped for disease in field and greenhouse conditions from 2016-2021.  Significant variation among rNILs and environments were detected. QTL for FHB resistance and DON accumulation were mapped in this population. Some lines exhibited lower disease severity than Gen1-001 and could be used as parents for breeding and further study.