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2021 National Fusarium Head Blight Forum


Gene Discovery & Engineering Resistance (GDER)

Poster # 119

Association Genetics of Fusarium Head Blight and Deoxynivalenol Resistance in Aegilops tauschii

Authors & Affiliations:

Rizky Pasthika Kirana1,6, Barbara Steiner1, Kumar Gaurav2, Sanu Arora2, Gerlinde Wiesenberger3, Maria Doppler4, Rainer Schuhmacher4, Sebastian Michel1, Marc Lemmens1, Gerhard Adam3, Brande BH Wulff2,5, Hermann Buerstmayr1
1. Institute of Biotechnology in Plant Production, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Austria 2. John Innes Centre, Norwich Research Park, Norwich, UK 3. Institute of Microbial Genetics, Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Austria 4. Institute of Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Austria 5. Center for Desert Agriculture, Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia 6. Laboratory of Plant Breeding, Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia

Corresponding Author:

Rizky Pasthika Kirana
University of Natural Resources and Life Sciences
pasthika@boku.ac.at

Abstract:

Aegilops tauschii is the diploid progenitor of the wheat D subgenome and known as a precious resource of genetic diversity for wheat breeding. To evaluate the potential of Ae. tauschii for Fusarium head blight (FHB) resistance, 147 Ae. tauschii accessions were phenotyped for resistance against Fusarium graminearum and its major mycotoxin deoxynivalenol (DON). At anthesis, the accessions were treated either with F. graminearum spores or pure DON solution, and the spreading of the disease or the toxin-induced bleaching were recorded. A k-mer-based association mapping pipeline based on whole-genome sequence data was used to dissect the genetic basis of FHB and DON resistance and to identify candidate resistance genes.The accessions revealed broad variation for FHB spreading within the spike controlled by many minor-effect QTL. For DON resistance, less variation was detected, comprising no highly resistant accessions, but nine accessions showed severe bleaching symptoms after DON infiltration concomitant with lower conversion rates of DON into the conjugated non-toxic DON-3-O-glucoside. Association genetics for DON resistance identified a uridine diphosphate (UDP)-glucosyltransferase (UGT) on chromosome 5D in the nine susceptible accessions with lower DON detoxification rates.Only the full-length UGT, which is also polymorphic in hexaploid wheat, conferred resistance against DON, confirming its prominent role in DON detoxification and thus in the Ae. tauschii/wheat-Fusarium-interaction.Aegilops tauschii, Fusarium head blight, deoxynivalenol, UDP-glucosyltransferase

Acknowledgment:

The Indonesia – Austria scholarship programm; Rizky Pasthika Kirana, was supported and funded by the Ministry of Education and Culture of the Republic of Indonesia and the Austrian Agency for International Cooperation in Education and Research (OeAD-GmbH) in cooperation with ASEA-UNINET

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