Globally, Fusarium head blight (FHB) is one of the most devastating fungal diseases of wheat. The use of resistant cultivars is the most effective way to reduce the adverse effects of FHB on yield and quality of wheat. However, the development of resistant cultivars has been a challenge for breeders since FHB resistance is a complex trait and only a few resistance sources are available. Hence, there is a constant need to identify new sources of resistance and we found HSD2-32 (2n=42) as a novel source of type II resistance to FHB in wheat. However, the complete information of the genetic markers linked with the genomic loci conferring FHB resistance in HSD2-32 is not available. Here we developed the F2 bi-parental population of HSD2-32 x Chinese Spring for QTL mapping. The F2 population was phenotyped for FHB resistance by point inoculation. Genotype-by-sequencing was used for genotyping the F2 population. Inclusive Composite Interval Mapping (ICIM) was done with Kosambi mapping function using QTL IciMapping Version 4.2 and linkage was considered to be established if a LOD score exceeds 3.0. We found putative QTLs in Chromosomes 2D, 4A, 4B,4D and 7A. A total of 54 markers were designed covering all the positions, out of which 8 chromosome specific markers were selected in 2D and 4A chromosomes that are linked with the QTL regions and these markers were validated using F3 contrast population. Only one SNP marker in Chr2D (Chr2D_627128588) segregate in 3:1 ratio. The experiment is progressing to develop more markers linked with these QTL regions having polymorphism across different genetic background. Also, the population is being forwarded to subsequent generations to develop recombinant inbreed lines (RILs) by single seed descent method.