Spray-induced gene silencing (SIGS) is a non-transgenic RNA interference (RNAi) strategy used for the control of various pathogen and pest. In this system, naked or nanomaterial-coated dsRNA is sprayed on plants to cause silencing of selective genes. Our aim is to use this strategy for the silencing of genes responsible for the growth and pathogenicity in Fusarium graminearum (Fg). The hypothesis is that the nanoparticle coating offers stability and slow-release to siRNAs or dsRNAs with continuous inhibition of Fg during the critical time of infection facilitating on-farm RNAi success. When treated with 20 mM siRNA oligo of Fg genes MGV1 and COT1 coated with chitosan nanoparticles, conidia of Fg failed to germinate. However, the blank controls with chitosan nanoparticles alone also displayed inhibitory effects on conidium germination in Fg. For MGV1, conidium germination rate was 2.1% at 6 h when siRNA was coated with Graphene quantum dots (GQDs). When blank GQDs without siRNA were used, the germination rate was 81.7%. For COT1, treatments with 25% siRNA oligo reduced over 60% germination rate at 6 h but treatments with 50% siRNA oligo blocked germination. Treatments with siRNA oligoes coated with GQDs targeting the PMK1 gene significantly reduced appressorium formation and virulence in the rice blast fungus, indicating that coating with GQDs may be suitable for other fungal pathogens. For wheat, we designed native and synthetic RNAi construct (200-300) bp partial coding region of the selected genes, including wheat phytoene desaturase (PDS), by using the pssRNAit web server. The dsRNA for PDS was produced using the double T7 plasmid L4440 harboring the native and synthetic RNA segments and transformed into HT115 (DE3) strain of E. coli which was induced to produce dsRNA using Isopropyl β-D-1-thiogalactopyranoside (IPTG). Preliminary results showed that GQDs-coated dsRNAs targeting PDS gene resulted in photobleaching phenotype, indicating the effects of siRNA on degrading the complementary mRNA sequences of the PDS gene. This gives the clues to move forward with the use of wide range of GQD concentrations for the improvement of dsRNA effectiveness and targeting Fg genes for managing FHB disease in wheat.