Enhancing genetic diversity for FHB resistance is critical for addressing the need for FHB-resistant wheat cultivars. Although many resistance QTL have been identified and deployed in US wheat cultivars, effective resistance against FHB is still not fully realized. It emphasizes the need for some innovative approaches to tackling this important disease. We have recently screened wild wheat germplasm from its D-genome as well as A-genome progenitor species to identify resistant novel genes and alleles. We have critically evaluated a large diverse triticale collection against FHB. In addition,  we have applied chemical and physical mutagens to develop mutant populations in diploid (Aegilops tauschii and Triticum monococcum) and polyploid wheat germplasm (including Shirley, Jagger, Corrigin, Chinese Spring, Hilliard, and MD131). We have also developed mutant populations of two winter barley cultivars adapted to MD and the broader mid-Atlantic region. Screening of some of these mutant populations in wheat allowed us to identify mutants with moderate to high resistance against FHB both for disease severity and DON content. Work is in progress on using MutMap and sequence-tagged high-resolution deletion mapping approaches to identify physical regions and associated genetic markers for the utilization of novel FHB resistance in breeding programs.