South Dakota ranks among the top ten barley-producing states in the US with 49,000 acres under cultivation, up 63% from 2021 (NASS 2022). While most of the cultivated barley feeds livestock, some of it goes to the brewing industries. The major threat to barley cultivation comes from Fusarium head blight disease (FHB, Scab), caused by the fungal pathogen Fusarium graminearum. FHB reduces the yield and quality by contaminating the produce with mycotoxins like deoxynivalenol (DON), negatively affecting the consumption of both Wheat and Barley worldwide. The most efficient way of controlling FHB is through imparting host resistance. Therefore, the first objective of our research is to screen spring barley cultivars against FHB disease. Winter hardiness in barley is a desirable trait that can provide South Dakota growers with an alternative to include barley in the crop rotation scheme. Barley, when included in the corn-soybean-small grain-cover crop rotation during winters reaps good returns to the growers while improving soil health and reducing the need for herbicide application. The second objective of our research is to identify winter hardy and FHB-resistant barley lines, which will provide both yield and sustainability benefits to the barley producers. We conducted field trials to test the FHB resistance of 23 and 29 winter barley lines and spring barley lines respectively. Out of the 23 winter barley lines received from different states of the United States, only 6 lines survived the winters of south Dakota. The 29 spring barley lines were planted in four replicates in a randomized complete block design and were spray inoculated at 50% anthesis stage with a virulent strain of Fusarium graminearum. The disease severity was rated by scoring the visible symptoms on heads at 21 days and 28 days after inoculation. At 21 days, the mean disease severity for the majority of the lines was below 40%, whereas at 28 days, two spring barley lines S2M196 and S2M197 performed significantly better exhibiting less than 40% severity in each block. The spring and winter barley samples will be proceeded for DON and FDK analysis. Our results show that variability for FHB resistance exists in the germplasm. Growers can make sound decisions by selecting moderately resistant to resistant varieties along with fungicide application against FHB as a disease management strategy.