Fusarium Head Blight (FHB) is the most devastating plant disease affecting barley (Hordeum vulgare) production in the east coast. While most frequently associated with Fusarium graminearum, recent studies in NY have shown multiple species associated with FHB in malting barley. Since 2008, demethylation inhibitors (DMI’s, FRAC code 3) have been the major class of fungicide active ingredients used to control FHB but concerns of fungicide resistance developing have prompted the registration of new fungicides with additional modes of action. In 2019, pydiflumetofen, a succinate dehydrogenase inhibitor (SDHI, FRAC code 7), was labeled for control of FHB in small grains. In effort to document baseline fungicide sensitivities in malting barley, isolates were collected in Delaware, Maryland, and New York during the 2021-22 growing seasons. Isolates were identified to species by amplification of Translation Elongation Factor 1ɑ (TEF1). In the preliminary set of 28 isolates, 79% were identified as F. graminearum, 7% F. verticilloides, 7% F. acuminatum, 3.5% F. poae, and 3.5% F. asiaticum. New York isolates were the most diverse containing four of the species recovered. An in vitro poison plate mycelial assay was conducted using yeast bacto agar (YBA) amended with pydiflumetofen to final concentrations of 0, 0.01, 0.05, 0.25, 1.0, and 5.0 μg/mL. A subset of F. graminearum isolates was also grown on PDA amended to the same concentrations for comparison of media types. The effective concentration to reduce mycelial growth by 50% (EC50) was determined for each isolate and averaged by species. Average EC50 values were 1.09 μg/mL for F. graminearum (n=22), 1.07 μg/mL for F. verticilloides (n=2), 1.51 μg/mL for F. acuminatum (n=2), 1.09 μg/mL for F. poae (n=1), and 1.09 μg/mL for F. asiaticum (n=1). Averages of F. graminearum on PDA were 1.04 μg/mL (n=8). Growth on YBA was more symmetric and this media will be used for all future studies. The baseline fungicide sensitivity levels documented in this preliminary work will be expanded and used to monitor the response of Fusarium spp. to SDHI fungicides as use expands.