The considerable increase of Deoxynivalenol (DON) levels was observed during the malting of FHB infected hard-red spring wheat in my previous research. In this study, the role of Fusarium damaged kernels (FDKs) was evaluated by separating the malting of FDKs and healthy wheat kernels. With the naturally FHB infected wheat, DON contents increased from 0.65±0.11 µg/g to 2.42±0.23 µg/g following malting. The content of DON increased from 2.96±0.43 µg/g to 19.38±4.26 µg/g during the malting of FDKs, but no significant (p>0.05) increase of DON contents (i.e. <0.50 µg/g) was found in the malting of healthy kernels. With the severely FHB infected wheat (i.e. wheat inoculated with F. graminearum using a grain spawn method in the field), DON contents increased from 4.22±0.55 µg/g to 18.55±4.07 µg/g following malting. The content of DON increased from 8.26±1.48 µg/g to 41.11±6.31 µg/g during the malting of FDKs, but no significant (p>0.05) increase of DON contents (i.e. <1.00 µg/g) was found in the malting of healthy kernels. The fungal hyphae were observed extremely heavy in the wheat and malt FDKs that contained high contents of Fusarium Tri5 DNA and DON (i.e. up to 276.72 µg/g), versus the relatively healthy kernels. The results indicated that wheat FDKs were responsible for the DON accumulation during malting, which associated with the interior infection of Fusarium hyphae within the kernels.