Prosaro® PRO 400 SC Fungicide is a new fungicide from Bayer Crop Science for broad-spectrum control of leaf and head diseases in small grain cereals. Prosaro® PRO was registered in the 3rd quarter of 2021 in several states of the United States and will be available for commercial use in 2022. Prosaro® PRO is a mixture of prothioconazole plus tebuconazole plus fluopyram. These three active ingredients provide overlapping control of key head and foliar diseases in cereal small grains. Prosaro® PRO provides increased control of Fusarium head blight and several important leaf diseases as well as suppression of ergot, compared to Prosaro®. Prosaro® PRO is registered for use in spring wheat, durum wheat, winter wheat, and barley. Prosaro® PRO is formulated as a soluble concentrate for ease of handling. Prosaro® PRO should be applied at 10.3 to 13.6 fl oz/ac with a non-ionic surfactant to wheat or barley, up to 30 and 32 days prior to harvest, respectively. For maximum performance of Prosaro® PRO in wheat, it is recommended to apply at flower initiation and up to 4-7 days later. For barley, it is recommended to apply Prosaro® PRO when barley heads are fully emerged on the main stems. Replicated trials were conducted at several locations throughout the United States from 2018 to 2021, with Prosaro® PRO at 10.3 fl oz/ac with a non-ionic surfactant at 0.125% v/v. Additionally, in 2018 to 2021, large demonstration trials were conducted by growers in commercial fields. The growers applied a tank mix treatment of Prosaro® plus Proline® plus Luna® Privilege at rates that delivered prothioconazole plus tebuconazole plus fluopyram equivalent to Prosaro® PRO at 10.3 fl oz/ac. These treatments were compared to either a non-treated check or Prosaro® at 6.5 to 8.2 fl oz/ac. The objective of these trials was to evaluate the influence of Prosaro® PRO on grain yield and grain quality in wheat and barley. Disease ratings were recorded where applicable. The trials were harvested, and grain yield and grain quality were determined.