Invited Presenter

Jin-Rong Xu
1. Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana
Corresponding Author: Jin-Rong Xu, jinrong@purdue.edu

Xu, Jin-Rong

jinrong@purdue.edu

Deoxynivalenol (DON), a type B trichothecene, is the most frequently detected mycotoxin in cereal grains. To better understand transcriptional regulation of DON biosynthesis in Fusarium graminearum, we conducted stranded RNA-seq analysis with the tri6 and tri10 mutants cultured under DON-inducing conditions and identified antisense transcripts of key TRI genes and a lncRNA of TRI5 transcribed from the promoter region of TRI5 (named RNA5P). The TRI10 and TRI6 transcription factor genes regulate each other’s expression. They both are involved in regulating the expression of TRI5 sense and antisense transcripts as well as RNA5P, which has cis-acting inhibitory effects on TRI5 expression and DON biosynthesis. We also characterized the functional relationship between Tri6 and Tri10 and the effects of chromatin modifications as global regulators on TRI gene expression. Results from these studies indicate that complex regulatory mechanisms are involved in controlling DON biosynthesis in F. graminearum.

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